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Fig. 5 | Journal of Translational Medicine

Fig. 5

From: Lactobacillus salivarius reverse antibiotic-induced lung defense impairment in a ventilator model

Fig. 5

ROS scavenger, NAC, treatment abolishes the stimulatory effect of FOS feeding on antibacterial protein expression of the intestinal mucosa and reversed the stimulatory effect of FOS on DCFDA levels of the intestinal mucosa and peroxynitrite production as well as the PA phagocytic activity of AMs. a Antibiotic treatment before MV significantly decreased the protein expression of CRP-ductin, IL-17, Reg3β, and RELMβ of the intestinal mucosa as compared with the control group. FOS feeding in mice receiving antibiotic treatment significantly increased the protein expression of CRP-ductin, IL-17, Reg3β, and RELMβ of the intestinal mucosa as compared with the antibiotic group. NAC treatment reversed the stimulatory effect of FOS treatment on protein expression of CRP-ductin, IL-17, Reg3β, and RELMβ of the intestinal mucosa. Data are presented as mean ± SEM. b NAC, a ROS scavenger, was orally given to mice after antibiotics with FOS treatment to examine the involvement of intestinal ROS production in lung defense mechanisms. Mice received intramuscular injection of antibiotics with FOS feeding and NAC feeding for 3 days. ROS levels in the intestinal mucosa were analyzed by DCFDA fluorescent dye. DCF is detected by fluorescence spectroscopy with excitation and emission spectra of 495 nm and 529 nm, respectively. Data are presented as mean ± SEM. c NAC was given to mice after antibiotic with FOS treatment and AMs of those mice were harvested for peroxynitrite assay. Data are presented as mean ± SEM. d NAC was given to mice after antibiotic with FOS treatment and AMs of those mice were harvested for the PA phagocytic activity assay. Data are presented as mean ± SEM; PA, P. aeruginosa; DCF, 2ʹ7ʹ-dichlorofluorescein; FOS, fructo-oligosaccharides; NAC, N-acetylcysteine. *P < 0.05, **P < 0.01. FOS, fructo-oligosaccharides; NAC, N-acetylcysteine. *P < 0.05, **P < 0.01

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