Table 5 GO enrichment analysis of (A) molecular functioning, (B) biological processes, and (C) cellular components for the DE genes involved in ARPE19-HBx and ARPE19-control cells, pre- and post-UV irradiation
Gene set name | Genes in overlap | P value |
|---|---|---|
(A) Molecular functions | Before UV irradiation | |
1. Testosterone dehydrogenase (NAD +) activity | 4 | 3.24E−04 |
2. Integrin binding | 9 | 1.93E−03 |
3. Extracellular matrix binding | 5 | 2.17E−03 |
4. Chemokine activity | 6 | 3.93E−03 |
5. Cytokine activity | 11 | 4.86E−03 |
6. Retinol dehydrogenase activity | 4 | 6.34E−03 |
7. Heparan sulfate proteoglycan binding | 4 | 6.34E−03 |
8. Heparin binding | 10 | 8.49E−03 |
9. Chemoattractant activity | 4 | 1.97E−02 |
10. MAP kinase tyrosine/serine/threonine phosphatase activity | 3 | 3.08E−02 |
Molecular functions | Post UV irradiation | |
1. Protein binding | 1142 | 5.27E−20 |
2. Chromatin binding | 94 | 6.79E−06 |
3. Single-stranded DNA-dependent ATPase activity | 9 | 9.70E−06 |
4. Single-stranded DNA binding | 30 | 6.45E−05 |
5. DNA binding | 311 | 2.13E−04 |
6. Transcription factor binding | 67 | 2.25E−04 |
7. DNA helicase activity | 12 | 3.65E−04 |
8. Double-stranded DNA binding | 26 | 6.50E−04 |
9. Insulin-like growth factor I binding | 8 | 7.38E−04 |
10. Transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding | 55 | 8.53E−04 |
(B) Biological processes | Before UV irradiation | |
Positive regulation of smooth muscle cell proliferation | 11 | 2.03E−06 |
Cellular response to tumor necrosis factor | 13 | 5.47E−06 |
Cellular response to interleukin-1 | 11 | 1.14E−05 |
Inflammatory response | 24 | 1.23E−05 |
Positive regulation of p38MAPK cascade | 6 | 1.72E−05 |
Cyclooxygenase pathway | 5 | 6.50E−05 |
Positive regulation of endothelial cell proliferation | 9 | 1.52E−04 |
Prostaglandin biosynthetic process | 5 | 1.87E−04 |
Cell adhesion | 24 | 2.02E−04 |
Negative regulation of cell proliferation | 22 | 2.67E−04 |
Biological processes | Post UV irradiation | |
DNA replication | 77 | 1.27E−22 |
Cell division | 114 | 2.75E−16 |
Mitotic nuclear division | 84 | 8.94E−13 |
G1/S transition of mitotic cell cycle | 44 | 5.69E−11 |
DNA replication initiation | 22 | 1.55E−10 |
DNA repair | 71 | 1.03E−08 |
Sister chromatid cohesion | 39 | 4.31E−08 |
DNA strand elongation involved in DNA replication | 13 | 4.52E−08 |
DNA synthesis involved in DNA repair | 20 | 9.88E−08 |
Telomere maintenance via recombination | 19 | 1.04E−07 |
(C) Cellular components | Before UV irradiation | |
Extracellular region | 66 | 6.60E−07 |
Extracellular space | 53 | 4.71E−05 |
Proteinaceous extracellular matrix | 16 | 8.91E−04 |
Endoplasmic reticulum | 33 | 1.27E−03 |
Integral component of plasma membrane | 49 | 1.39E−03 |
Synaptic vesicle | 9 | 1.41E−03 |
Extracellular matrix | 15 | 6.08E−03 |
Plasma membrane | 110 | 9.46E−03 |
Organelle membrane | 7 | 1.04E−02 |
Anchored component of membrane | 8 | 1.11E−02 |
Cellular components | Post-UV irradiation | |
Nucleoplasm | 580 | 7.09E−20 |
Cytoplasm | 941 | 1.79E−12 |
Nucleus | 957 | 1.10E−10 |
Nuclear matrix | 35 | 1.30E−06 |
Cytosol | 598 | 2.40E−06 |
Chromatin | 32 | 7.57E−06 |
Centrosome | 99 | 8.62E−06 |
Nuclear chromosome, telomeric region | 40 | 9.80E−06 |
Nuclear envelope | 45 | 3.66E−05 |
Replication fork | 11 | 3.98E−05 |