Fig. 4

The caspase-3 dependent apoptosis at 48 h after reperfusion from each group. a–c Effects on TUNEL staining in the ventral grey matter. d–f Effects on immunoreactivity to caspase-3 in the ventral grey matter. g Number of TUNEL positive cells in the ventral grey matter. h Number of caspase-3 positive cells in the ventral grey matter. All data are presented as mean ± SEM (n = 8). Scale bar = 200 μm. **P <0.05 versus Sham; ^##P < 0.05 versus I/R