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Table 1 Primers and short hydrolysis probes used for TETs mRNA expression analysis

From: In vivo evidence of ascorbate involvement in the generation of epigenetic DNA modifications in leukocytes from patients with colorectal carcinoma, benign adenoma and inflammatory bowel disease

Gene Forward primer sequence Reverse primer sequence UPL Probe sequence
TET1 5′-TCTGTTGTTGTGCCTCTGGA-3′ 5′-GCCTTTAAAACTTTGGGCTTC-3′ #57 GGCCCCAG
TET2 5′-GCCTTTGCTCCTGTTGAGTT-3′ 5′-ACAAGGCTGCCCTCTAGTTG-3′ #38 GGAAGCAG
TET3 5′-CACTCCGGAGAAGATCAAGC-3′ 5′-GGACAATCCACCCTTCAGAG-3′ #1 CCTGGAGC
TBP 5′-GAACATCATGGATCAGAACAACA-3′ 5′-ATAGGGATTCCGGGAGTCAT-3′ #87 CTGCCACC
HMBS 5′-TGCCCTGGAGAAGAATGAAG-3′ 5′-CAGCATCATGAGGGTTTTCC-3′ #69 CTTCCTCC