Table 1 Primers and short hydrolysis probes used for TETs mRNA expression analysis
Gene | Forward primer sequence | Reverse primer sequence | UPL | Probe sequence |
|---|---|---|---|---|
TET1 | 5′-TCTGTTGTTGTGCCTCTGGA-3′ | 5′-GCCTTTAAAACTTTGGGCTTC-3′ | #57 | GGCCCCAG |
TET2 | 5′-GCCTTTGCTCCTGTTGAGTT-3′ | 5′-ACAAGGCTGCCCTCTAGTTG-3′ | #38 | GGAAGCAG |
TET3 | 5′-CACTCCGGAGAAGATCAAGC-3′ | 5′-GGACAATCCACCCTTCAGAG-3′ | #1 | CCTGGAGC |
TBP | 5′-GAACATCATGGATCAGAACAACA-3′ | 5′-ATAGGGATTCCGGGAGTCAT-3′ | #87 | CTGCCACC |
HMBS | 5′-TGCCCTGGAGAAGAATGAAG-3′ | 5′-CAGCATCATGAGGGTTTTCC-3′ | #69 | CTTCCTCC |