Fig. 6
From: Resibufogenin suppresses colorectal cancer growth and metastasis through RIP3-mediated necroptosis
Resibufogenin RIP3-dependently suppresses heterotropic CRC growth. a HCT116 Cells were pretreated with NSA (1 μM) for 8 h prior to a 24 h treatment with resibufogenin. HCT116 + NSA, HCT116 shControl and HCT116 shRIP3 cells viability was measured by MTT assay (n = 6) b and the proportion of PI-positive cells was analyzed by flow cytometry (n = 3). c Western blotting experiments for RIP3, MLKL, P-MLKL (S358) were performed with the cell lysates obtained after resibufogenin treatment in HCT116 shControl and HCT116 shRIP3 cells. d The picture of colon tumor tissue in mice. e Isolated tumor size and tumor weight from the HCT116 shControl and HCT116 shRIP3 mice heterotropic CRC model