Table 1 Proportion of major cell types detected in live and fixed PBMC
From: PBMC fixation and processing for Chromium single-cell RNA sequencing
Donor ID | PBMC processing | Number of cells captured | Myeloid cells | Lymphoid cells | |||
|---|---|---|---|---|---|---|---|
Dendritic Cells | Monocytes | B cells | T cells | NK cells | |||
DTM-X | Live + PBS | 4975 | 105 (2.11%) | 1442 (28.98%) | 230 (4.62%) | 2774 (55.76%) | 365 (7.34%) |
DTM-X | Methanol-3H + SSC | 2933 | 56 (1.91%) | 818 (27.89%) | 175 (5.97%) | 1622 (55.30%) | 242 (8.25%) |
DTM-X | Methanol-3W + SSC | 2826 | 50 (1.77%) | 783 (27.71%) | 196 (6.97%) | 1513 (53.54%) | 228 (8.07%) |
DTM-Y | Live + PBS | 5883 | 141 (2.40%) | 1815 (30.85%) | 473 (8.04%) | 2900 (49.29%) | 354 (6.02%) |
DTM-Y | Methanol-3W + SSC | 3899 | 64 (1.64%) | 982 (25.19%) | 358 (9.18%) | 2165 (55.53%) | 272 (6.98%) |