Fig. 6
From: Novel distance-progesterone-combined selection approach improves human sperm quality
Effect of selection combined-distance-progesterone on sperm apoptosis. A Sperm nucleus (red); B annexin V on the surface of sperm (apoptotic, green); C late (yellow arrow) and early (red arrow) apoptotic sperm; D nonapoptotic sperm (pink arrow). I The bar represents 5 μm. II The percentage of apoptotic sperm after different treatments. Data are expressed as mean ± SEM (n = 6). There was no significant difference between sperm treated with Percoll and sperm not treated with Percoll (independent samples t test, P = 0.993), or between positions D2 and E2 (P = 0.464). Non-Percoll indicates the sperm were not processed by Percoll before selection; Percoll indicates sperm were processed by Percoll before selection; D2 and E2, position of device. Min, minutes; P, P value