Fig. 3

Morphology, identification of PKH67-labeled BMSCs, characterization and FACS analysis of undifferentiated BMSC surface markers. A, B The morphology of BMSCs at passages 0 (A) and 3 (B). C Before transplantation, cells were marked with the fluorescent membrane-intercalated dye PKH67. D, E The BMSCs differentiated into the adipogenic and osteogenic lineages. F, G Flow cytometry analysis of the immune phenotype of cultured cells. The cells were CD29(+), CD90(+), CD45(−), CD34(−), with partial expression of CD73 and CD44. These data indicated that they were of mesenchymal stem cell lineage. Values are the means ± SDs. Scale bar, 200 μm (A– C), 100 μm (D and E)