Skip to main content
Fig. 5 | Journal of Translational Medicine

Fig. 5

From: Inhibition of endoplasmic-reticulum-stress-mediated autophagy enhances the effectiveness of chemotherapeutics on pancreatic cancer

Fig. 5

TUNEL staining showing synergy of sunitinib and gemcitabine in apoptosis of human PDAC cells. a Miapaca-2 were cultured in 4-well sterile borosilicate chambered cover glass and treated for 48 h with gemcitabine (250 nM), sunitinib (10 µM), and chloroquine (25 µM), or in combinations. Cells were fixed and processed for TUNEL and DAPI staining and were visualized using a fluorescent microscope. The combination treatment shows significantly increased TUNEL positive cells compared to the single treatment groups. b Bar-charts showing mean percentage of TUNEL positive cells in each treatment groups (n = 5). The combination treatments result in significantly increased TUNEL positive cells for Sun + CQ (p < 0.05), Gem + CQ (p < 0.05), Sun + Gem (p < 0.01) and Sun + Gem + CQ treatments (p < 0.01). *p < 0.05, **p < 0.01, Scale bar, 5 µM, CQ chloroquine, Gem gemcitabine, Sun sunitinib

Back to article page