Fig. 4

Ultrastructural pathology of sunitinib treated human PDAC cells. Panc3.27 and Miapaca-2 cells were treated with DMSO or sunitinib, 10 µM for 72 h and fixed in glutaraldehyde/PFA and processed for electron microscopy. TEM images of DMSO (left) and sunitinib-treated (right) Panc3.27 cells (top panel) and Miapaca-2 cells (bottom panel) showing organelle pathology of ER vesicles (arrowhead), and lysosomes (arrow). Sunitinib treated cells exhibit enlarged ER lumen with granular materials and have large multivesicular lysosomal bodies in the cytoplasm surrounding the nucleus. Scale bar, 500 nM. CQ chloroquine, Gem gemcitabine, Sun sunitinib