Fig. 3

Increased KLF4 expression in the nucleus led to cetuximab resistance. a Immunofluorescence confirmed that KLF4 was highly expressed in the cytoplasm of in Klf4-del NES cells, and KLF4 was overexpressed in the nucleus when KLF4 was locally expressed in the nucleus of Klf4-del NLS cells. b The nuclear and cytoplasmic proteins in different HONE1 cells were extracted separately, and the expression of KLF4 protein in the nucleus and cytoplasm was verified by Western blot. c The Klf4-del NES 5–8F and HONE1 cells were treated with cetuximab, and the apoptosis was affected significantly, meanwhile the apoptosis of cKLF4-HONE1 cells was significantly reduced after treatment with cetuximab (d)