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Fig. 5 | Journal of Translational Medicine

Fig. 5

From: Lapine periodontal ligament stem cells for musculoskeletal research in preclinical animal trials

Fig. 5

Multi-differentiation potential of PDL cells (microscopic examination) a PDL cells were grown in osteogenic differentiation media for 4 weeks and then stained with ARS. Red color represented mineralized nodules after PDL cells differentiated to osteoblast like cells. b PDL cells were grown in neurogenic differentiation media for 3 weeks and then stained with Cresyl violet stain. Change in morphology represented differentiation of PDL cells to neuronal like cells. c PDL cells were grown in AIM and AMM and stained with ORO. Large number of orange lipid vacuoles were seen in both rPDL cells and hPDL cells. d PDL cells were grown in chondrogenic differentiation media for 4 weeks. H & E staining revealed dark blue differentiated nuclei representing chondrocyte like cells in both rPDL cells and hPDL cells as compared to controls

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