Fig. 5
HER2 imaging in tumors and organs of NCI-N87- and MKN45-induced xenograft mice. a The HER2 imaging detection in NCI-N87 and MKN45 xenograft model. Both NCI-N87 and MKN45 cells (2 × 106) were subcutaneously inoculated into the right flank of nude mice for establishing a HER2 high- and low-expressed xenograft model, respectively. The Cy5.5-conjugated AHNP-PEG was intravenous injected into mice for 6 h and detected by using IVIS. b The distribution of HER2 imaging in tumors and various organs (brain, heart, lung, liver, spleen, kidney, stomach, colon, and muscle) of NCI-N87 xenograft mice. The Top panel showed the HER2 imaging in various organs. Button panel showed the quantitation of fluorescent signals. c The protein expressions of HER2 in tumors and various organs of NCI-N87 xenograft mice. Protein expressions were determined by Western blotting. All data are presented as mean ± SEM (n = 3). d The HER2 expressions in tumors of NCI-N87- and MKN45-induced xenograft mice. Protein expressions were determined by Western blotting and quantified by densitometry and normalized by GAPDH levels. All data are presented as mean ± SEM (n = 3). *P < 0.05, versus MKN45-induced xenograft mice