Fig. 3

AHNP-PEG decreased cell viability in HER2-overexpressed GC cells. a The protein expressions of HER2 in GC cells (NCI-N87 and MKN45) and human primary stomach epithelium cells (HPSEC) were shown. Protein expressions were determined by Western blotting and quantified by densitometry and normalized by GAPDH levels. The data are presented as mean ± SEM (n ≥ 3). *P < 0.05, versus MKN45 cells. b The effect of AHNP-PEG on cell viability. The NCI-N87, MKN45 and HPSEC cells were treated with AHNP-PEG (0–100 μg/ml) for 24 and 48 h. Data are presented as mean ± SEM (n = 5). *P < 0.05, versus control; NS non-significant. c The effect on cell viability between AHNP-PEG and FITC-AHNP-PEG. The NCI-N87, MKN45, and HPSEC cells were treated with AHNP-PEG and FITC-AHNP-PEG (0–100 μg/ml) for 24 h. Data were presented as mean ± SEM (n = 5). *P < 0.05, versus control; NS non-significant