Fig. 5
Human serum ELISA using XC24p11-STA to detect anti-SF3B1 autoantibody effectively distinguished between HCC and non-HCC samples. a Detection of anti-SF3B1 autoantibody using XC24p11-STA in sera from patients with HCC. The specific binding of autoantibody against XC24p11 epitope was calculated by the difference between the optical density (OD) value of XC24p11-STA ELISA and that of Eph-STA ELISA. Non-HCC (n = 85), HCC (n = 102). AFP was also quantified in the same sample sets using human AFP Quantikine ELISA Kit (R&D systems) and the diagnostic efficiency was evaluated by ROC curve analysis. These experiments were repeated three times and representative results are shown. CV cutoff value, SE sensitivity, SP specificity; b Anti-XC24p11-STA ELISA depending on tumor stage, tumor size, and viral infection (HBV or HCV). The upper panel shows the anti-XC24p11 antibody reactivity (ODXC24p11) in each serum sample and the lower panel shows the percentage of each group above or below the CV. The clinical information of HCC patients is summarized in Table S1. The dotted line on the plot represents the CV and the short line in red represents the mean value of each group