Fig. 4
From: Granulin epithelin precursor promotes colorectal carcinogenesis by activating MARK/ERK pathway
GEP knockdown results in G1 arrest and apoptosis in CRC. a Accumulation of G1 cells increased and S-phase cell percentage decreased in siGEP transfectants compared with siControls in DLD-1cell and HCT 116 cells lines. b The percentage of early apoptotic cells in siGEP treated cells was significantly increased compared to the siControl cells in these two cell lines. c Western blot of CyclinD1, activation of cleaved Caspase 3, activation of cleaved Caspase 8 and cleaved PARP expression after silencing GEP in DLD-1 and HCT 116 cells. d Relative luciferase reporter activity of cell cycle signaling shown in GEP suppressed DLD-1 and HCT 116 cells. e RNA expression of GEP was positively associated with CyclinD1 in TCGA database. (*P < 0.05; **P < 0.01)