Fig. 6

viSNE on antigen-specific cells finds differential markers. Boolean ‘OR’ gates for CD107a, IFNγ, TNFα and IL-4 from responders (n = 14) and non-responders (n = 5) were concatenated in Flowjo v10.1 for both CD4 and CD8 T cells after RSV (F) peptide stimulation at day 0 and day 8, and visualized in t-SNE space with viSNE software in Cytobank. viSNE analysis on antigen-specific T cells showed high expression of HLA-DR, CCR7, CD69 and CD127 in non-responders compared to responders at day 0 and day 8 in CD4 T cells (a). The same trend is seen in CD8 T cells (b) using this approach. Cells are colored by median intensity levels from high (red) to low (blue)