Table1 Primers used for qRT-PCR
Gene | Forward primer sequence (5′–3′) | Reverse primer sequence (5′–3′) |
|---|---|---|
Runx2 | 5′ AGA CCA GCA GCA CTC CAT AT 3′ | 5′ CTC ATC CAT TCT GCC GCT AGA 3′ |
Alp | 5′ ATG GCT CAC CTG CTT CAC G 3′ | 5′ TCA GAA CAG GGT GCG TAG G 3′ |
Bmp2 | 5′ ATG GGT TTG TGG TGG AAG TG 3′ | 5′ TTG GCT TGA CGC TTT TCT CG 3′ |
Vegf | 5′ AGG AGT ACC CCG ATG AGA TA 3′ | 5′ CTT CTA CTG CCC TCC TTG TA 3′ |
Ocn | 5′ AGG GCA GTA AGG TGG TGA AT 3′ | 5′ GCA TTA ACC AAC ACG GGG TA 3′ |
Gapdh | 5′ GGCACAGTCAAGGCTGAGAATG3 | 5′ ATGGTGGTGAAGACGCCAGTA3′ |