Skip to main content
Fig. 6 | Journal of Translational Medicine

Fig. 6

From: Nanoparticle delivery of grape seed-derived proanthocyanidins to airway epithelial cells dampens oxidative stress and inflammation

Fig. 6

Effect of SLN-GSE on NF-κB nuclear translocation mediated by H2O2. H441 cells were incubated with three different doses of SLN-GSE and free GSE 24 h (a), 48 h (b), and 72 h (c) before treatment with H2O2, then immunodetection of NF-κB was performed, and the nuclear:cytoplasmic fluorescence ratio was calculated. Data are  shown as mean ± standard deviation of three experiments conducted each in duplicate. *p < 0.05 **p < 0.01 ***p < 0.001 for H2O2 vs all conditions. d Representative images of NF-κB signal (green) and nuclear signal (blue) obtained with untreated cells (CTRL), treated with H2O2, or incubated with the lowest doses (2.5 µM) of SLN-GSE and free GSE for 72 h before H2O2. Cells in the free GSE panel with NF-κB nuclear staining only are indicated by white arrows. Images were obtained with an original magnification of ×20 and after zooming (×4). Scale bar: 20 µm

Back to article page