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Fig. 5 | Journal of Translational Medicine

Fig. 5

From: CD8+ T cells mediate the antitumor activity of frankincense and myrrh in hepatocellular carcinoma

Fig. 5

CD8+ T cells mediate antitumor activity of FM in vivo. 4- to 6-week-old male C57BL/6 mice received subcutaneous injection of 5 × 106 Hepa1-6 cells. When tumor volumes reached about 4–6 mm in diameter, mice were randomized to four groups. Anti-CD8 or anti-NK neutralizing antibodies were injected intraperitoneally (500 μg/mouse) 1 day prior to and 13 days after FM treatment (60 mg/kg/day, n = 8). Mice in other two groups received oral gavage of FM alone (60 mg/kg/day, n = 8), or left untreated (n = 8). a The scheme depicts the schedules of the interventions. b Peripheral blood was obtained from each mouse, stained with anti-CD8-PerCp or anti-NK1.1-PerCp antibodies and then subjected to flow cytometry to monitor the depletion of CD8 or NK cells 1 and 13 days after first injection of neutralizing antibodies. ce Tumor volume and body weight were monitored every 3 days during the treatment. c Tumor volume of each mouse in each group is shown at each monitoring time point. d An overview of tumor growth e and body weight are also shown. Mean ± SD of each group are shown. #p > 0.05, *p < 0.05, **p < 0.01, ****p < 0.0001

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