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Fig. 1 | Journal of Translational Medicine

Fig. 1

From: CD8+ T cells mediate the antitumor activity of frankincense and myrrh in hepatocellular carcinoma

Fig. 1

FM reduces the activation of NF-κB and STAT3 in HCC cells. a Human HCCLM3 or mouse Hepa1-6 HCC cells were seeded in 96-well plates overnight. Then cells were treated with serial concentrations of FM. 24 and 48 h later, cells were subjected to MTT assay. be 2 × 105 HCCLM3 or Hepa1-6 cells were seeded into 12-well plates. Cells were treated with FM at indicated concentrations for 24 h and then stimulated with recombinant TNF-α (human, 15 ng/μl; mouse, 80 ng/μl) or recombinant IL-6 (both at 25 ng/μl). Total protein or mRNA was extracted and subjected to western blot or qRT-PCR analysis. b The protein level of total/phosphorylated IκBα, c mRNA level of TNF-α and IL-6, and d protein level of total/phosphorylated STAT3 after TNF-α stimulation are shown. GAPDH was used as loading control. e Phosphorylation of STAT3 after IL-6 stimulation is shown. The results of qRT-PCR are shown as Mean ± SD. Similar results were obtained in three independent experiments. #p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

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