Fig. 9

Additive effects of 5-FU and T7 − MB on T cells using the therapeutic schedule (n ≥ 5 mice/group). a Cytotoxic T cell responses were induced by 5-FU and T7 − MB, as indicated by the lysis of EAC cells using the LDH method. b Increases in CD8⁺ and CD4⁺ T cells in splenic lymphocytes were induced by 5-FU and T7 − MB, as determined by flow cytometry. c Flow cytometry results of CD8⁺ T cells in splenic lymphocytes in the PBS control (left) and 5-FU and T7 − MB combination (right) groups. d Flow cytometry results of CD4⁺ T cells in splenic lymphocytes in the PBS control (left) and 5-FU and T7 − MB combination (right) groups. The data represent the mean ± SE; *P < 0.05 and **P < 0.01 compared to the PBS control group