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Fig. 5 | Journal of Translational Medicine

Fig. 5

From: Connexin32 plays a crucial role in ROS-mediated endoplasmic reticulum stress apoptosis signaling pathway in ischemia reperfusion-induced acute kidney injury

Fig. 5

Cx32 gene deletion mitigated I/R-induced ROS generation and ERS activation. a, b Renal I/R induced the alteration of Cx32 protein expression was detected by western blotting analysis. n = 6, *p < 0.05 vs Sham group. c Cx32 gene deletion significantly alleviated ROS production and distribution (magnification ×200). df Cx32 gene deletion significantly alleviated I/R (24 h)-induced GRP78, XBP1and CHOP expression increase. Changes of GRP78, XBP1and CHOP expressions were determined by western blotting analysis. Immunofluorescence staining (magnification ×400; red: GRP78 and XBP1; green, CHOP; blue: the nuclear labeled by DAPI) and immunohistochemistry (magnification ×400)

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