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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: CCL20 is up-regulated in non-alcoholic fatty liver disease fibrosis and is produced by hepatic stellate cells in response to fatty acid loading

Fig. 4

Transcript levels of CCL20 and CCR6 in HepG2 (a, b) and LX-2 (c, d) cells in response to fatty acid loading. a CCL20 transcript levels were increased by nearly fivefold (**Mann–Whitney p = 0.0029) in HepG2 cells treated with 1 mM oleic acid compared with BSA treated control cells, and by just over two- and threefold (*Mann–Whitney p < 0.0001), respectively, by 250 and 500 mM palmitic acid. b In contrast, in LX-2 cells, CCL20 levels increased ~ 13-fold with 250 μM (p < 0.0001) and the positive control LPS (p < 0.0001), ~ 15-fold with 500 μM of palmitic acid (p < 0.0001), and ~twofold (p < 0.0001) and ~eightfold (p < 0.0001) in response to oleic acid or 1 mM palmitic acid/oleic acid (50/50) mixture, respectively. c No statistically significant differences were observed in CCR6 transcript levels across the different treatments in HepG2 cells after adjusting for multiple comparisons. d In LX-2 cells, the only pairwise comparison to achieve statistical significance was ~ 50% decrease in CCR6 transcript levels in response to 500 μM oleic acid

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