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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: Postprandial increase in serum CA125 as a surrogate biomarker for early diagnosis of ovarian cancer

Fig. 4

Glucose and insulin-invoked synthesis, secretion and re-absorption of CA125 in cancer cell models. a The time-dependent fluctuations of extracellular and intracellular CA125 levels and Mucin 16/ACTB mRNA ratios after administration of saline, glucose, insulin and glucose plus insulin in SKOV-3, HO8910, ES-2 and OVCAR-3 cancer cell lines, respectively (note: Mucin 16/ACTB mRNA ratios were set to 1 for saline controls). b The time-dependent re-absorption of Alexa Fluor 488-labeled CA125 in cultured ovarian cancer cells (SKOV-3) under saline, high-glucose, insulin-only, high-glucose plus insulin or CA125-deglycosylation conditions. The per cell fluorescent particles were presented as the mean of the particles counted in 20 randomly selected ovarian cancer cells under a ×200 microscopic field. The addition of insulin (i.e., the insulin-only and high-glucose plus insulin groups) significantly accelerate re-absorption of CA125 protein (green particles) into the cytoplasm of cultured cells, while the deglycosylation of CA125 protein partially blocked the re-absorption-promoting effect of insulin. *Statistical significance (ANOVA)

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