Table 1 Sequences of primers for qRT-PCr in this study
From: Role of p53/miR-155-5p/sirt1 loop in renal tubular injury of diabetic kidney disease
Gene | Sequences | |
|---|---|---|
Sirt1 | Sense | 5′-AGTTCCAGCCGTCTCTGTGT-3′ |
Antisense | 5′-CTCCACGAACAGCTTCACAA-3′ | |
Atg5 | Sense | 5′-TGTGCTTCGAGATGTGTGGTT-3′ |
Antisense | 5′-ACCAACGTCAAATAGCTGACTC-3′ | |
Atg7 | Sense | 5′-ACCCAGAAGAAGCTGAACGA-3′ |
Antisense | 5′-CTCATTTGCTGCTTGTTCCA-3′ | |
Col-1 | Sense | 5′-TCAAGACACGTTCCCGTGAG-3′ |
Antisense | 5′-GCCAACTTCTCCAGCGGTA-3′ | |
GAPDH | Sense | 5′-GAACGGGAAGCTCACTGG-3′ |
Antisense | 5′-GCCTGCTTCACCACCTTCT-3′ | |
miR-155-5p | Sense | 5′-GCCTCCAACTGACTCCTACA-3′ |
Antisense | Universal reverse primer (Tiangen, Beijing, China) | |
U6 | Sense | 5′-CTCGCTTCGGCAGCACA-3′ |
Antisense | Universal reverse primer (Tiangen, Beijing, China) | |