Fig. 4

The Sirt1 regulate expression of P53. a Sirt1 mRNA expression was detected by qRT-PCR analysis in HK-2 cells transfected with pCMV-Sirt1 plasmid, and confirmed by d western blot with e quantitative analysis. b Sirt1 over expression increased the levels of Atg5, Atg7, LC3 II and decreased the expression levels of P53, P62, FN, Col-1 by qRT-PCR and h western blot with i quantitative analysis. a Sirt1 mRNA expression was detected by qRT-PCR analysis in HK-2 cells transfected with si-Sirt1, and confirmed by f western blot, g quantitative analysis. c Inhibition of expression of Sirt1 gene increased the levels of P53, P62, FN, Col-1 and decreased the expression levels of Atg5, Atg7, LC3 II by qRT-PCR and j western blot with k quantitative analysis. The experiments were performed in triplicate. The data are expressed as the mean ± SED. *P < 0.005. pCMV-Sirt1 Sirt1 over expression, pCMV-control Sirt1 over expression negative control