Fig. 2

MiR-155-5p promote Sirt1-inhibited fibrosis in HK-2 cells via inhibiting autophagy activity. a miR-155-5p mRNA expression was detected by qRT-PCR analysis in HK-2 cells transfected with miR-155-5p mimics/inhibit. b–e miR-155-5p inhibit decreased the expression levels of Sirt1, Atg5, Atg7, Col-1, on the contrary, miR-155-5p inhibit increased the expression levels of Sirt1, Atg5, Atg7, Col-1 by qRT-PCR and f the influence of miR-155-5p mimics/inhibitor on Sirt1, LC3II and p62, FN, Col-1 expression level measured by western blot with (f–i) quantitative analysis. Results are presented as mean ± SEM of three independent experiments. *P < 0.05. miR-NC miRNA negative control, miR-155-5p-m miR-155-5p mimics, miR-155-5p-i miR-155-5p inhibitor