Table 1 Primers used for real-time PCR

GAPDH

Forward: 5′-GGAGAAAGCTGCTAA-3′

Reverse:5′-ACGACCTGGTCCTCGGTGTA-3′

Runx2

Forward: 5′-TATGAAAAACCAAGTAGCAAGGTTC-3′

Reverse: 5′-GTAATCTGACTCTGTCCTTGTGGAT-3′

Osteocalcin

Forward: 5′-GTGCAGAGTCCAGCAAAGGT-3′

Reverse: 5′-CTAGCCAACTCGTCACAGTC-3′

Col2A1

Forward: 5′-CAGGCAGAGGCAGGAAACTAAC-3′

Reverse: 5′-CAGAGGTGTTTGACACGGAGTAG-3′

Sox9

Forward: 5′-GTACCCGCACCTGCACAAC-3′

Reverse: 5′-TCCGCCTCCTCCACGAAG-3′

PPARγ

Forward: 5′-GACCACTCCCACTCCTTTGA-3′

Reverse: 5′-CGACATTCAATTGCCATGAG-3′

LPL

Forward: 5′-TACAGGGCGGCCACAAGTTTT-3′

Reverse: 5′-ATGGAGAGCAAAGCCCTGCTC-3′