Fig. 3

CF33 induces immunogenic cell death in pancreatic cancer cells. PANC-1 and MIA PaCa-2 cells were mock-infected or infected with CF33 at an MOI of 5. Cell surface-exposed CRT was quantified by flow cytometry (a), and the release of ATP was measured using ATP assays (b) at 16 h post infection. The release of HMGB1 was analyzed by Western blot at 0, 24, 48, and 72 h post infection (c). Data represent the mean ± SD, **p < 0.01