Fig. 2

In vitro cytotoxicity and viral replication of chimeric orthopoxvirus CF33-Fluc in pancreatic cancer cell lines. Viability of pancreatic cancer cell lines BxPC-3, PANC-1, MIA PaCa-2, SU.86.86, AsPC-1 and Capan-1 was assessed daily for 8 days after infection with CF33-Fluc. Percent cell survival over time is shown at MOI^† = 1 (a), MOI = 0.1 (b), and MOI 0.01 (c). Dose-dependent cell killing was seen in all cell lines. d Viral proliferation was inversely correlated to the LD50^‡ in PANC-1, SU.86.86, AsPC-1 and Capan-1 (R² = 0.932). (^†MOI, multiplicity of infection. ^‡LD50, lethal dose for 50% cell killing)