Skip to main content

Advertisement

Fig. 2 | Journal of Translational Medicine

Fig. 2

From: Cardiopulmonary bypass reduces myocardial oxidative stress, inflammation and increases c-kit+CD45 cell population in newborns

Fig. 2

Cardiac cell turnover was not affected by cardiopulmonary bypass. The percentage of Ki67+ cycling cardiomyocytes (a) and non-cardiomyocytes (c) was not affected by cardiopulmonary bypass both in newborns (n = 4) and children (n = 7). The apoptotic rates of cardiomyocytes (f) and non-cardiomyocytes (h) was similar in both groups before and after cardiopulmonary bypass. There was no statistical difference in the percentage of Ki67+ cycling cardiomyocytes (b) and non-cardiomyocytes (d) and in the percentage of apoptotic cardiomyocytes (g) and non-cardiomyocytes (i) in biopsies from atrium and ventricle of children (n = 3). Representative sections from the atrium of a newborn before and after cardiopulmonary bypass demonstrating co-immunostaining for proliferation marker Ki67 (red) and cardiomyocyte marker alpha-sarcomeric actin (green) (e). Nuclei are stained blue by DAPI. Bars = 10 µm. Representative sections from the atrium of a newborn before and after cardiopulmonary bypass demonstrating light microscopic staining for apoptosis (brown) (j). Bars = 30 µm

Back to article page