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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: The positive role of vitronectin in radiation induced lung toxicity: the in vitro and in vivo mechanism study

Fig. 4

VTN modulated the expression level of fibrosis members in vivo. ac The relative mRNA expression levels of VTN (a), collagen I (b), collagen III (c) and α-SMA (d) in the lung tissues of mice from different groups were analyzed via qRT-PCR, n = 6, *p < 0.05, **p < 0.01, ***p < 0.001 vs the sc-RNA group (n = 6 mice/group). eh The protein concentrations of VTN (e), Collagen I (f), Collagen III (g), and Hydroxyproline (h) in the lung tissues of mice from different groups were analyzed by ELISA, n = 6, *p < 0.05, **p < 0.01, ***p < 0.001 vs the sc-RNA group (n = 6 mice/group). i The immunohistochemical staining for VTN and collagen I in the lung tissue of mice from different groups; the staining results were imaged in presence of red (VTN), green (collagen I) and blue (nuclei). j The protein expression levels of collagen I, collagen III, VTN, and α-SMA in lung tissue of mice from different groups were analyzed by western blot analysis, GAPDH was used to confirm the equal amount of proteins loaded in each lane. The experiments described above were repeated three times at least, and their final data was expressed as mean ± SD

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