Fig. 2

VTN regulated the expression levels of collagen I and III. a The transfection efficiency of si-RNA 1 (targeted VTN), si-RNA 2 (targeted VTN) and si-RNA 3 (targeted VTN) were analyzed by quantitative RT-PCR, n = 3, ***p < 0.001 vs the si-NC (the control siRNA to the siRNA targeted VTN) group. b The relative mRNA expression levels of VTN in control group and OE-VTN group were analyzed by quantitative RT-PCR, n = 3, ***p < 0.001 vs control. c The protein expression level of VTN in the si-NC group, si-RNA groups was analyzed by western blot analysis. d The protein expression level of VTN in control group and OE-VTN group were analyzed by western blot analysis. e The concentrations of VTN in each group were analyzed by ELISA, n = 3, *p < 0.05, ***p < 0.001 vs the normal group. f The concentrations of collagen I in each group were analyzed by ELISA, n = 3, ***p < 0.001 vs the normal group. g The concentrations of collagen III in each group were analyzed by ELISA, n = 3, ***p < 0.001 vs the normal group. h The protein expression levels of VTN, collagen I and III in different groups were analyzed by western blot analysis. β-actin was used to confirm the equal amount of proteins loaded in each lane. The experiments described above were repeated three times at least, and their final data was expressed as mean ± SD