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Fig. 1 | Journal of Translational Medicine

Fig. 1

From: The positive role of vitronectin in radiation induced lung toxicity: the in vitro and in vivo mechanism study

Fig. 1

Expression level of VTN increased in irradiated WI-38 and IMR-90 cell lines. a, b The protein expression levels of VTN at different time after irradiated with different doses in WI-38 (a) and IMR-90 cell lines (b) were analyzed by ELISA, n = 3, *p < 0.05 vs 0 Gy group. c, d The mRNA expression levels of VTN, collagen I and III at different time points after 8 Gy irradiation in WI-38 (c) and IMR-90 cell lines (d) were analyzed by quantitative RT-PCR, n = 3, *p < 0.05 vs 0 h in post-irradiation group. e, f The protein expression levels of VTN, collagen I and III at different time points after 8 Gy irradiation in WI-38 (e) and IMR-90 cell lines (f) were analyzed by western blot analysis, β-actin was used to confirm the equal amount of proteins loaded in each lane. The experiments described above were repeated three times at least, and their final data was expressed as mean ± SD

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