Fig. 5
From: Association between serum S100A9 levels and liver necroinflammation in chronic hepatitis B
Elevated S100A9 expression in liver tissues and cells infected with HBV. a IHC staining of S100A9 in representative biopsied liver samples from CHB patients as well as the liver samples from HCs. Blank scale bars = 100 µm. b real-time PCR analysis of S100A9 gene in liver normal L02 cells transfected with and without pcDNA3.1-HBV or its control pcDNA3.1 for 24 h. c Western blot analysis of S100A9 expression in L02 cells transfected with and without pcDNA3.1-HBV or its control pcDNA3.1 for 48 h. d Statistical densitometric ratios of c. e Immunofluorescence staining for S100A9 in L02 cells transfected with and without pcDNA3.1-HBV or its control pcDNA3.1 for 48 h. White scale bars = 100 µm. f ELISA analysis for S100A9 levels in L02 cells transfected with and without pcDNA3.1-HBV or its control pcDNA3.1 for 48 h. Data represents the mean ± SD, *p < 0.05; **p < 0.01