Fig. 1

Representative double-labeled immunofluorescence identification of TCs. Images of c-kit negative staining were not shown; Scale bar = 50 μm. a Positive FITC labelling for Vimentin (green). b Positive CY3 labelling for CD34 (red). Nuclei were counterstained with DAPI (blue). c In the merged image, Vimentin and CD34 were overlapped, both in the cellular body and full length of Tp, with characteristic podomer (thin segments) and podom (thick segments) arrayed alternatively along Tp