Fig. 1

Treatment with periostin inhibits  suture-derived cell proliferation and migration. a The cell viabilities of suture-derived cells treated with periostin in different concentrations were measured with a CCK-8 assay at days 1, 3, 5, and 7. b, c The suture-derived cells were grown to a confluent monolayer, wounded by manual scratching with a sterile pipette tip, and subjected to further incubation with periostin. The scratched areas filled by migrated suture-derived cells were observed at 24 h post-scratching and quantified using Image-Pro Plus software. Original magnifications: ×40. Bar: 100 μm. d, e As determined by transwell assay, the migratory suture-derived cells were visualized by imaging the nuclei labeled with DAPI. The number of migrated cells was counted in five randomly selected fields. Original magnifications: ×40. Bar: 100 μm. *P < 0.05, **P < 0.01 compared to 0 μg/L group