Fig. 5

STAT3 mediates EMT reversal induced by YM155. a Western blot analysis of pSTAT3 (Ser727), pSTAT3 (Tyr705), STAT3, E-cadherin, and N-cadherin in U251 and U87 cells before and after knockdown with STAT3 siRNA. b Morphology of U251 and U87 cells before and after STAT3 siRNA knockdown. c Crystal violet staining of Transwell Matrigel assay for U251 and U87 cells control or transfected with STAT3 siRNA after radiation treatment. d Quantitation of migrating cell number in experiments from (c). e Western blot analysis of pSTAT3 (Ser727), pSTAT3 (Tyr705) and STAT3 in lysates prepared from control cells and cells stably expressing STAT3. f Crystal violet staining of Transwell Matrigel assay from control or stable ectopic expression of STAT3 U251 cells after control, radiation (4 Gy), YM155 (5 nM) and combination treatment (5 nM YM155 + 4 Gy radiation). g Quantitation of migrating cell number in experiments from (f). h Crystal violet staining of Transwell Matrigel assay from YM155 treated U251 and U87 cells or stable ectopic expression of STAT3 U251 and U87 cells following YM155 treatment. i Quantitation of migrating cell number in experiments from (h). **P < 0.01; ***P < 0.001; size bars = 50 µm