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Fig. 3 | Journal of Translational Medicine

Fig. 3

From: YM155 decreases radiation-induced invasion and reverses epithelial–mesenchymal transition by targeting STAT3 in glioblastoma

Fig. 3

YM155 decreases invasion induced by radiation and reverses EMT in GBM cells. a Representative images of Transwell Matrigel assays from U251 and U87 cells in DMSO, radiation, and combination treatment (YM155 5 nM + radiation 4 Gy) fixed and stained with crystal violet. b Quantitation of migrated cell numbers from (a). c Morphology of U251 and U87 cells under bright field microscopy after treatment with vehicle or YM155. d Representative fluorescence images of phalloidin staining of DMSO and YM155 (5 nM) treated U251 and U87 cells. e Western blot analysis of E-cadherin, N-cadherin, β-catenin, Zeb1, and slug in U251 and U87 cells incubated with increasing concentrations of YM155. **P < 0.01; ***P < 0.001; size bars = 50 µm

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