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Fig. 6 | Journal of Translational Medicine

Fig. 6

From: Neonatal Fc receptor is involved in the protection of fibrinogen after its intake in peripheral blood mononuclear cells

Fig. 6

FcRn expression in diverse cell lineages. a RT-PCR analysis for the expression of FcRn at transcript level in Jurkat, SH-SY5Y and T-cells. b Western blot analysis for the expression of FcRn at protein level in Raji, Jurkat, U-937, THP-1 and SH-SY5Y cell lines. c Deglycosylation by PNGase F treatment on total protein lysates from SH-SY5Y cells. d SH-SY5Y cells were incubated with fibrinogen-FITC (0.4 mg/ml in serum-free medium) for 4 h. Cells were then washed 3 times and analyzed by flow cytometry at time zero (T0) and after 2 h (T2h). Solid histograms indicate the fibrinogen-FITC loaded cells, whereas the dashed histograms indicate the unloaded cells

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