Fig. 1

Fibrinogen expression in PBMCs. a A representative western blot analysis of a total cell lysate of non-T and T-cell fraction of PBMCs from the same individual (male, aged 51) and of Jurkat cell line. b Fibrinogen β-chain (FGB) mRNA expression in PBMCs and T-cells (from the same individual) and in hepatocarcinoma HepG2 cells (as positive control). After total RNA extraction, reverse transcription and semi-quantitative PCR, the amplification products (FGB and GAPDH as housekeeping control) were separated on a 2% agarose gel stained with ethidium bromide