Fig. 3

γ-secretase inhibitor DAPT decrease the mRNA expression of Notch1 and its target gene Hes-1. Mice were euthanized after 7 consecutive days of experimental process, and RNA samples from splenic single-cells was used for detection. Both the mRNA expression of Notch1 (a) and Hes-1 (b) was obviously enhanced in IMQ-treated mice compared to control mice (^#P < 0.01). In IMQ + DAPT-treated mice, Notch1 and Hes-1 mRNA expression was significant decrease (^#P < 0.01). Splenic CD4⁺ T cells from 8 IMQ-treated mice were collected and treated with DAPT, Notch1 (c) and Hes-1 (d) mRNA expression presented a dramatically reduced trend with the increased concentration of DAPT (^#P < 0.01)