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Fig. 1 | Journal of Translational Medicine

Fig. 1

From: Effect of γ-secretase inhibitor on Th17 cell differentiation and function of mouse psoriasis-like skin inflammation

Fig. 1

γ-secretase inhibitor DAPT Alleviates the Severity of IMQ-Induced Psoriasis-like Skin Inflammation BALB/c mice were treated daily with IMQ cream (IMQ-treated and IMQ + DAPT-treated groups) or control cream (vaseline group) applied on their shaved back skin. In IMQ + DAPT-treated group, in addition to the topical IMQ application, the mice were co-treated daily with intraperitoneal injection of γ-secretase inhibitor DAPT (10 mg/kg/day). In control and IMQ-treated mice, they received an equal amount of DMSO. After 7 consecutive days of experimental process, mice were euthanized. A Phenotypical presentation of mouse back skin after 7 days of treatment. Control mice (a) did not present any sign of inflammation. IMQ-treated mice (b) presented significant inflammation changes of erythema, scaling and thickening. The severity of inflammation was obviously alleviated in IMQ + DAPT-treated mice (c). B Erythema, scale, and thickness were scored daily on a scale from 0 to 4: 0, none; 1, slight; 2, moderate; 3, marked; 4, very marked. The cumulative score was calculated by scores of erythema plus scale plus thickness (scale 0–12). Each score of IMQ-treated mice was higher than that of control mice (#P < 0.01), while, after DAPT and IMQ co-treatment, all of these scores were reduced (#P < 0.01). C Pathological examination of mouse back skin after 7 days of treatment observed by haematoxylin and eosin (HE) stain. The epidermis layer of control mice (a) was thin and composed by only 1–2 layers of epidermal cells. IMQ-treated mice (b) presented obviously epidermal hyperplasia with hyperkeratosis, parakeratosis, Munro microabscess and elongation of trochanterellus as well as massive dermal infiltration of inflammatory cells with dilated capillaries. The severity of epidermal hyperplasia and dermal inflammatory cells infiltration were significantly reduced in IMQ + DAPT-treated mice (c)

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