Fig. 8
Increased cytokine production by CD8 TEM cells expressing integrin α4β7 among CVD 1208S vaccinees. a Identification of CD8 TEM cells expressing integrin α4β7. b Gating example to evaluate the expression of IFN-γ and TNF-α in integrin α4β7− and integrin α4β7+ CD8 TEM cells among individuals who exhibited increased production of these cytokines after the first immunization (day 28). The data shown in b are from volunteer #24. c Formula used to calculate the ratio (R) of cytokine production (IFN-γ and TNF-α) among integrin α4β7− and integrin α4β7+ CD8 TEM cells. c Also displays an example of the calculations using data from volunteer #24, i.e., each of the 3 quadrants (Q1, Q2 and Q3) containing single or double positive cells). d A summary of the data in integrin α4β7− and integrin α4β7+ in CD8 TEM cells from the vaccinated individuals that increased TNF-α and IFN-γ 28 days after the first vaccination