Fig. 5
Multifunctional responses induced by CVD 1208S in TEM cells 28 days after the first immunization. a, e The comparison of cell that showed a single function (S+) (e.g., cells producing one cytokine or upregulating CD107a alone) versus multifunctional (MF) cells (e.g., cells exhibiting more than one function) in CD8 and CD4 TEM cells, respectively. b, f The comparison of cells that showed 2–5 simultaneous functions (2+, 3+, 4+ and 5+). For each cytokine, S+ and MF cells were compared. c CD8 TEM MF cells that produced TNF-α (in any combination) were more abundant than those producing TNF-α alone. These results were not observed in CD4 TEM cells (g). The arithmetic mean ± SD is indicated in all the groups. d, h The relative frequency (expressed as percentage) of the 5 most prevalent MF populations in CD8 TEM and CD4 TEM cells, respectively. The remaining MF populations were pooled in a single group indicated as other. *P < 0.05 (Mann–Whitney)