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Fig. 2 | Journal of Translational Medicine

Fig. 2

From: RPE phagocytic function declines in age-related macular degeneration and is rescued by human umbilical tissue derived cells

Fig. 2

Phagocytosis level in RPE from eyes of AMD donors. RPE cells were isolated from eyes of 4 AMD donors with age 65, 84, 86, and 88, and cultured as described in “Methods”. For best possible age matching RPE isolated from 3 normal donors without ocular diseases of age 61, 71 and 79 were used as normal control. 5 × 104 human RPE cells are plated in a 24-well plate in MEM containing 20% (v/v) FBS for a week followed by medium change to MEM with 5% (v/v) FBS and subjected to phagocytosis assay. Normal RPE cells from eyes of donors without ocular diseases, AMD RPE cells from eyes of donors with AMD. Data represent the mean ± SEM (n = 16). ****P < 0.0001. Representative images of RPE containing fluorescent ingested POS are shown (scale bar = 10 µm)

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