Fig. 4From: The role of atorvastatin in suppressing tumor growth of uterine fibroidsEffect of atorvastatin on apoptosis in human uterine fibroids cells. Immortalized human uterine fibroids cells were treated with increasing concentrations of atorvastatin (0, 1, 5, 10, 20 and 40 μM) for 48 h (a) or 72 h (b), and then followed by flow cytometry after Annexin-V FITC Staining. Data (apoptosis rate) presented were mean ± SEM from triplicate measurements (c). *P < 0.05 with one-way ANOVA. Immortalized human uterine fibroids cells (d) and primary uterine fibroids cells (e) were treated with increasing concentrations of atorvastatin (0, 1,5, 10, 20 and 40 μM) for 48 h (immortalized cells) or 72 h (primary cells) and then followed by western blot analysis. Tubulin was used as the loading control. The intensity of each protein band was quantified and normalized with corresponding tubulin, and the normalized values were used to generate the graphs. Data presented were mean ± SEM from triplicate measurements. *P < 0.05 with one-way ANOVABack to article page