Fig. 4

Validation of differentially expressed mRNAs in the blood of MFS patients (n = 26) compared to HV controls (n = 26) as determined by RT-qPCR (P < 0.05). Mean ΔCt MFS and HV controls (Lower ΔCt, higher expression level). β-Actin as an endogenous housekeeping gene for normalization, Unpaired-two-tailed t tests and ± standard deviation (STDV) were used to evaluate differences in expression. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001