Fig. 5

miR-8084 enhances the proliferation and epithelial-to-mesenchymal transition. a Protein levels of ING2 in BT-549^NC, BT-549^miR-8084, MDA-MB-231^NC and MDA-MB-231^{miR-8084 inhibitor} cells were detected by western blotting. b Relative protein levels of proliferation related markers (AKT, ERK1/2, PCNA and Ki-67) in BT-549^NC, BT-549^miR-8084, MDA-MB-231^NC and MDA-MB-231^{miR-8084 inhibitor} cells were analyzed by western blotting. c Cell proliferation was measured by the MTS assay. BT-549 cells were transfected with miR-8084 mimics or control, at 24 h post-transfection, the MTS assay was performed every 24 h for 4 days (*P < 0.05). d MDA-MB-231 cells were transfected with miR-8084 inhibitor or control, at 24 h post-transfection, the MTS assay was performed every 24 h for 4 days (*P < 0.05). e EMT-like morphology of BT-549 cells transfected with miR-8084 mimics or control. f EMT-like morphology of MDA-MB-231 cells transfected with miR-8084 inhibitor or control. g Relative protein levels of EMT markers (epithelial cell marker E-cadherin and the mesenchymal cell markers N-cadherin, vimentin and snail) in BT-549^NC, BT-549^miR-8084, MDA-MB-231^NC and MDA-MB-231^{miR-8084 inhibitor} cells were analyzed by western blotting